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Updated: Jun 19, 2026

Imaging Mismatch Repair and Cellular Responses to DNA Damage in Bacillus subtilis
Published on: February 8, 2010
Tn917 targets the region where DNA replication terminates in Bacillus subtilis, highlighting a difference in
Qiaojuan Shi1, Jose C Huguet-Tapia, Joseph E Peters
1Department of Microbiology, Cornell University, Ithaca, NY 14853, USA.
Abstract:
The bacterial transposon Tn917 inserts preferentially in the terminus region of some members of the Firmicutes. To determine what molecular process was being targeted by the element, we analyzed Tn917 target site selection in Bacillus subtilis. We find that Tn917 insertions accumulate around the central terminators, terI and terII, in wild-type cells with or without the SPbeta lysogen. Highly focused targeting around terI and terII requires the trans-acting termination protein RTP, but it is unaffected in strains compromised in dimer resolution or chromosome translocation. This work indicates that Tn917 is sensitive to differences in DNA replication termination between the Firmicutes.
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