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Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm
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Broomrape can acquire viruses from its hosts.

Amit Gal-On1, Anna Naglis, Diana Leibman

  • 1Department of Plant Pathology and Weed Science, Volcani Center, ARO, Bet Dagan. amitg@volcani.agri.gov.il

Phytopathology
|October 14, 2009
PubMed
Summary

Parasitic broomrape plants can host plant viruses like Cucumber mosaic virus (CMV). This study confirms CMV replicates within Phelipanche aegyptiaca, demonstrating broomrape as a host for viral pathogens.

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Area of Science:

  • Plant Pathology
  • Virology
  • Parasitic Plant Biology

Background:

  • Broomrapes (Phelipanche spp.) are parasitic plants that connect to host vascular systems via haustoria.
  • Plant viruses are known pathogens affecting numerous plant species.

Purpose of the Study:

  • To investigate the susceptibility of Phelipanche aegyptiaca to infection by Cucumber mosaic virus (CMV), Tomato mosaic virus (ToMV), Potato virus Y (PVY), and Tomato yellow leaf curl virus (TYLCV).
  • To determine if these viruses, particularly CMV, can replicate within the parasitic broomrape tissues.

Main Methods:

  • Infection assays using Phelipanche aegyptiaca grown on hosts infected with CMV, ToMV, PVY, and TYLCV.
  • Detection of viral RNA (plus and minus strands), siRNAs, coat proteins, and viral DNA in Phelipanche tissues using molecular techniques.
  • Purification and quantification of viral particles from infected broomrape.

Main Results:

  • CMV replicated in Phelipanche aegyptiaca, with accumulation of genomic RNA, siRNAs, and viral particles.
  • Viable ToMV and PVY were detected, along with ToMV RNA, but with low/undetectable coat protein levels.
  • Genomic TYLCV DNA was detected in Phelipanche shoots.

Conclusions:

  • Phelipanche aegyptiaca serves as a host for Cucumber mosaic virus (CMV), supporting its replication.
  • Broomrape plants can be infected by and potentially support the replication of other viruses like ToMV, PVY, and TYLCV.
  • This study establishes broomrape as a host for plant viruses, opening new avenues for research on plant-parasite-virus interactions.