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Related Concept Videos

M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...

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Related Experiment Video

Updated: Jun 19, 2026

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Recent developments in cyclin-dependent kinase biochemical and structural studies.

Aude Echalier1, Jane A Endicott, Martin E M Noble

  • 1Laboratory of Molecular Biophysics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK. aude.echalier-glazer@cbs.cnrs.fr

Biochimica Et Biophysica Acta
|October 14, 2009
PubMed
Summary
This summary is machine-generated.

This review examines cyclin-dependent kinases (CDKs) and their regulation. It highlights alternative CDK-cyclin complexes and factors influencing substrate specificity and inhibition.

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Related Experiment Videos

Last Updated: Jun 19, 2026

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
10:54

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations

Published on: September 17, 2012

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
13:38

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET

Published on: January 27, 2012

Area of Science:

  • Molecular Biology
  • Cellular Regulation
  • Structural Biology

Background:

  • Cyclin-dependent kinases (CDKs) are crucial regulators of cellular processes like proliferation and transcription.
  • Previous structural studies of CDK2 complexes established a model for CDK regulation.
  • Recent structural data necessitate a re-evaluation of this general model.

Purpose of the Study:

  • To review alternative CDK-cyclin assemblies, exemplified by CDK9/cyclin T1 and CDK4/cyclin D.
  • To examine the impact of CDK phosphorylation on activation and substrate specificity.
  • To elucidate the mechanisms of CDK regulation by Cip/Kip inhibitors.

Main Methods:

  • Review of recent structural and biochemical studies on CDK complexes.
  • Comparative analysis of different CDK-cyclin interactions.
  • Examination of phosphorylation effects on CDK activity.

Main Results:

  • Alternative CDK-cyclin complexes, such as CDK9/cyclin T1 and CDK4/cyclin D, offer new insights into CDK regulation.
  • CDK phosphorylation differentially affects activation states and substrate specificity across various CDK complexes.
  • Cyclin subunits significantly contribute to CDK substrate specificity.

Conclusions:

  • The general model of CDK regulation requires revision based on new structural data.
  • Understanding CDK-cyclin interactions and regulatory factors is key to deciphering cellular control mechanisms.
  • The complex roles of Cip/Kip inhibitors in CDK regulation are being unraveled.