Related Experiment Video
Updated: Jun 19, 2026
12:18
An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
Published on: July 22, 2011
Development and evaluation of a multiplex polymerase chain reaction assay to identify Salmonella serogroups and
Nora Cardona-Castro1, Miryan Sánchez-Jiménez, Lelia Lavalett
1Laboratorio de Microbiología, Instituto Colombiano de Medicina Tropical - Universidad CES Cra 43 A #52 Sur 99, Medellín, Colombia. ncardona@ces.edu.co
Diagnostic Microbiology and Infectious Disease
|October 14, 2009
Abstract:
To improve limitations of Salmonella serotyping, 2 multiplex polymerase chain reaction (M-PCR) were developed using a strategy that identifies first the genes encoding serogroups (rfbJ, wzx). According to the serogroup determined, a second M-PCR identifies serotype (fliC, fljB, wcdB, and sdf-I sequence). Standardization and evaluation of both M-PCRs were carried out.
