Regulation of TRH release by the cultured neonate rat pancreas
A Dutour1, P Giraud, J Y Maltese
1Laboratoire de Neuroendocrinologie Expérimentale, INSERM U 297, Faculté de Médecine Nord Bd P. Dramard, Marseille, France.
Abstract:
The TRH secretory responsiveness of the pancreatic islet cell clusters from newborn rat in organ culture was studied. Basal TRH secretion was stable over a 9-day period. The response to various secretagogues was tested on day 4. TRH secretion was stimulated by high potassium-induced depolarization and also through both cAMP and protein kinase-C dependent pathways. Like insulin, TRH release was stimulated by glucose and arginine and inhibited by somatostatin. These data suggest the existence of a common mechanism for TRH and insulin secretion by the pancreatic beta-cells.
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