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Updated: Jun 19, 2026

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

Published on: August 11, 2020

Simultaneous mechanical-scan-free confocal microscopy and laser microsurgery.

Kevin K Tsia¹, Keisuke Goda, Dale Capewell

¹Electrical Engineering Department, University of California, Los Angeles, CA 90095, USA.

|October 14, 2009

Summary

This study presents a novel single-fiber device for simultaneous confocal microscopy and laser microsurgery. This technology enables precise imaging and surgery without probe or sample movement, advancing minimally invasive procedures.

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Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks

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Area of Science:

Biomedical Engineering
Optical Imaging
Surgical Technology

Background:

Current endoscopic tools often lack the precision required for delicate surgeries.
Minimally invasive surgery demands smaller, more flexible, and highly accurate instrumentation.

Purpose of the Study:

To develop an endoscope-compatible device for simultaneous confocal microscopy and laser microsurgery.
To enable high-precision 2D imaging and microsurgery without mechanical probe or sample manipulation.

Main Methods:

A single-fiber-based optical system was engineered.
The method maps 2D sample coordinates to the optical spectrum.
This approach facilitates integrated imaging and surgical capabilities.

Main Results:

Demonstrated simultaneous confocal microscopy and laser microsurgery using a single fiber probe.
Achieved 2D imaging and microsurgery without mechanical scanning.
The device is compatible with endoscopic applications.

Conclusions:

The developed technology offers a pathway to highly miniaturized endoscopes.
This innovation is particularly promising for neurosurgery, pediatric surgery, and endovascular interventions.
The system's non-mechanical operation enhances precision and flexibility for complex surgical tasks.