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Updated: Jun 19, 2026

Rapid In Vitro Cytotoxicity Evaluation of Jurkat Expressing Chimeric Antigen Receptor using Fluorescent Imaging
Published on: October 27, 2023
Cytotoxic effect of potato aspartic proteases (StAPs) on Jurkat T cells
Julieta R Mendieta1, Carmela Fimognari, Gustavo R Daleo
1Institute of Biological Research, University of Mar del Plata, Mar del Plata, Argentina.
Abstract:
StAPs are potato aspartic proteases with cytotoxic activity against plant pathogens and spermatozoa. StAPs cytotoxic activity is selective, since these proteins do not exert toxic effect on plant cells and erythrocytes. In this work, we investigated the capacity of StAPs to exert cytotoxicity on human leukaemia cells. Obtained results show that StAPs induce apoptosis on Jurkat T cells after a short time of incubation in a dose-dependent manner. However, no significative effect on the T lymphocytes viability was observed at all StAPs incubation times and concentrations tested. These results suggest that StAPs can be conceptually promising leads for cancer therapy.
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