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A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
Published on: February 16, 2014
Identification of the amino acid-AZT-phosphoramidase by affinity T7 phage display selection
Jilin Cheng1, Xin Zhou, Tsui-Fen Chou
1Department of Medicinal Chemistry, College of Pharmacy, University of Minnesota, Minneapolis, MN 55455, USA.
Bioorganic & Medicinal Chemistry Letters
|October 15, 2009
Abstract:
A CEM cell cDNA T7 phage display library was prepared and used to screen for activating enzymes of phosphoramidate prodrugs of AZT monophosphate. Although, inefficient compared to ribonucleotide based phosphoramidates, hHint 1 was identified as the likely intracellular pronucleotide activating enzyme.
