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Related Concept Videos

Cellulose and Pectic Polysaccharides01:15

Cellulose and Pectic Polysaccharides

Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth.  Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds.
As a cell matures, its cell wall specializes according to its type. For example, the parenchyma cells of...
Cell Adhesion in Plants01:14

Cell Adhesion in Plants

Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose, and...
Plant Cell Wall01:07

Plant Cell Wall

Plant cells have a cell wall, a rigid outer covering that protects the cell and provides shape and support. During cell division, a mixture of enzymes, proteins, and glucose molecules is transported via vesicles to the center of the cell. These vesicles continuously fuse and build a cell plate between the dividing cells. As the cell plate matures, new polysaccharides are added to it to form the cell walls of the daughter cells. The predominant polysaccharide in the cell wall is cellulose, made...
Plant Cell Wall02:43

Plant Cell Wall

The plant cell wall gives plant cells shape, support, and protection. As a cell matures, its cell wall specializes according to the cell type. For example, the parenchyma cells of leaves possess only a thin, primary cell wall.Collenchyma and sclerenchyma cells, on the other hand, mainly occur in the outer layers of a plant's stems and leaves. These cells provide the plant with strength and support by either partially thickening their primary cell wall (i.e., collenchyma), or depositing a...
Role of Microtubules in Cell Wall Deposition01:02

Role of Microtubules in Cell Wall Deposition

Microtubules are small hollow tubes in eukaryotic cells. The cell wall microtubules are polymerized dimers of two globular proteins, α-tubulin and β-tubulin, two globular proteins. With a diameter of about 25 nm, microtubules are the widest components of the cytoskeleton. They help the cell resist compression and provide a track along which vesicles move through the cell or pull replicated chromosomes to opposite ends of a dividing cell. Microtubules go through quick cycles of disassembly and...
Chemistry of Carbohydrates03:25

Chemistry of Carbohydrates

Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...

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Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
11:06

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

Published on: October 16, 2017

Microanalysis of plant cell wall polysaccharides.

Nicolai Obel1, Veronika Erben, Tatjana Schwarz

  • 1Max-Planck-Institut for Molecular Plant Physiology, Am Mühlenberg 1, 14476 Golm, Potsdam, Germany.

Molecular Plant
|October 15, 2009
PubMed
Summary

Oligosaccharide Mass Profiling (OLIMP) offers a rapid and sensitive method for analyzing plant cell wall structures. This technique reveals significant heterogeneity in cell wall polymers across various plant tissues and cell types.

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Glycan Profiling of Plant Cell Wall Polymers using Microarrays
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OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides

Published on: June 20, 2010

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Last Updated: Jun 19, 2026

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
11:06

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

Published on: October 16, 2017

Glycan Profiling of Plant Cell Wall Polymers using Microarrays
12:30

Glycan Profiling of Plant Cell Wall Polymers using Microarrays

Published on: December 17, 2012

OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides
08:43

OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides

Published on: June 20, 2010

Area of Science:

  • Plant Biology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Plant cell walls are complex structures crucial for plant development and function.
  • Understanding cell wall polymer heterogeneity is key to deciphering plant growth and responses.
  • Existing methods for cell wall analysis can be time-consuming and require large sample amounts.

Purpose of the Study:

  • To introduce and validate Oligosaccharide Mass Profiling (OLIMP) coupled with MALDI-TOF MS for plant cell wall analysis.
  • To demonstrate the capability of OLIMP in revealing cell wall polymer heterogeneity.
  • To showcase OLIMP's application in analyzing single cell types and in situ tissues.

Main Methods:

  • Oligosaccharide Mass Profiling (OLIMP) combined with Matrix Assisted Laser Desorption Ionisation Time Of Flight Mass Spectrometry (MALDI-TOF MS).
  • Analysis of cell wall polymers including xyloglucan and homogalacturonan.
  • Application of laser micro-dissection for single cell type analysis.
  • In situ analysis of wall polymers on unprepared plant tissue.
  • Analysis of Golgi-enriched fractions for studying polysaccharide biosynthesis.

Main Results:

  • OLIMP provides fast and sensitive assessment of plant cell wall polymer structure.
  • High heterogeneity in the substitution patterns of xyloglucan and homogalacturonan was observed across different plant organs and cell types.
  • Successful analysis of xyloglucan structure in specific leaf cell types (epidermis, mesophyll, vascular bundles).
  • Demonstrated feasibility of in situ wall analysis and analysis of newly synthesized polysaccharides.
  • Enabled semi-quantitative analysis of cell wall composition.

Conclusions:

  • OLIMP is a powerful tool for detailed plant cell wall structural analysis.
  • The method's sensitivity and speed allow for high-resolution studies of cell wall heterogeneity.
  • OLIMP facilitates the investigation of cell wall biosynthesis and metabolism at an unprecedented level.