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Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
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Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level
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Diffusion processes of single fluorescent molecules in a polymer-based thin material with three-dimensional network.

Syoji Ito1, Takatsugu Kusumi, Satoshi Takei

  • 1Division of Frontiers Materials Science and Center for Quantum Science and Technology under Extreme Conditions, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka, Japan. sito@chem.es.osaka-u.ac.jp

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Summary

Single-molecule imaging shows polymer network formation affects guest dye mobility hierarchically. Initially, translation slows while rotation continues, with increasing crosslinking further restricting movement until both stop.

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Area of Science:

  • Polymer Science
  • Materials Science
  • Physical Chemistry

Background:

  • Polymer network formation involves crosslinking, altering material properties.
  • Understanding molecular mobility within polymer films is crucial for material performance.
  • Guest molecule dynamics can report on the evolving polymer matrix.

Purpose of the Study:

  • To investigate the hierarchical changes in guest dye mobility during polymer network formation.
  • To correlate guest dye diffusion and rotation with the degree of crosslinking.
  • To elucidate the distinct stages of mobility restriction in thin polymer films.

Main Methods:

  • Utilizing single-molecule imaging techniques.
  • Monitoring the translational and rotational diffusion of guest dye molecules.
  • Analyzing mobility changes as a function of reaction progress and crosslinking.

Main Results:

  • Hierarchical mobility changes observed: translational diffusion inhibited first, rotational diffusion remained active.
  • Fraction of translationally immobilized dyes increased with crosslinking.
  • At the final stage, both translational and rotational diffusion ceased.

Conclusions:

  • Polymer network formation progressively restricts guest molecule mobility in a hierarchical manner.
  • Translational mobility is more sensitive to early-stage crosslinking than rotational mobility.
  • Single-molecule imaging provides detailed insights into dynamic processes within forming polymer networks.