Highly selective detection of Cu2+ utilizing specific binding between Cu-demetallated superoxide dismutase 1 and the Cu2+ ion via surface plasmon resonance spectroscopy
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Suseung Lee1, Inhee Choi, Surin Hong
1School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University, Seoul 151-744, Korea.
Abstract:
A highly specific interaction between a metal-deficient metalloenzyme and metal ion has been utilized in the selective detection of the metal ion by surface plasmon resonance spectroscopy (SPRS). The use of SPRS and Cu-demetallated superoxide dismutase (E,Zn-SOD1) as a sensing actuator allows one to selectively and in situ detect Cu2+ without any interference that other spectroscopic methods may have.
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