Nanostructure-biomolecule interactions: implications for tissue regeneration and nanomedicine

Great strides are being made worldwide in our ability to synthesize and assemble nanoscale building blocks to create advanced materials with novel properties and functionalities. The novel properties of nanostructures are derived from their confined sizes and their very large surface-to-volume ratios. Nanostructured surfaces have also been shown to elicit more favorable and selective biomolecule and cellular responses than surfaces at coarser length scales. In the case of nanoscale ceramics and osteoblasts, for example, the benefit results from protein (vitronectin) unfolding at the nanostructured surface. These nanoscale attributes are enabling a variety of nanostructures to form the bases for a new field--nanomedicine. A fundamental issue in much of nanomedicine, and especially tissue regeneration, is to understand and to eventually control nanostructure-biomolecule interactions. To elucidate the fundamental bases for changes of protein conformation and function on nanostructured surfaces, and hence select responses including those of stem cells, a number of model experiments have been carried out. The results of these studies are presented and discussed in the context of the fundamental driving forces for protein conformation changes associated with nanostructures, their relationship to modified cell responses and tissue engineering, and our present knowledge regarding nanostructure properties.