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Surface characterization of extracellular matrix scaffolds.
Bryan N Brown1, Christopher A Barnes, Rena T Kasick
1Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA 15219, USA.
Biomaterials
|October 16, 2009
Summary
Extracellular matrix (ECM) scaffolds from different tissues have unique surface properties. Chemical cross-linking alters these characteristics, impacting scaffold design for tissue engineering.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Cell Biology
Background:
- Extracellular matrix (ECM) scaffolds influence cell behavior and phenotype.
- The relative importance of ECM molecular composition versus ultrastructure in determining cell phenotype remains unclear.
- Scaffold surface characteristics dictate host cell interactions and responses upon implantation.
Purpose of the Study:
- To investigate the surface characteristics of ECM scaffolds from porcine urinary bladder, small intestine, and liver.
- To evaluate the impact of two common chemical cross-linking methods on urinary bladder matrix (UBM) surface properties.
Main Methods:
- Scanning electron microscopy (SEM) for ultrastructural analysis.
- Time of flight secondary ion mass spectroscopy (ToF-SIMS) for molecular composition analysis.
- Characterization of native ECM scaffolds and chemically cross-linked UBM.
Main Results:
- ECM scaffolds exhibit distinct morphologic and structural properties based on their tissue origin.
- Chemical cross-linking significantly alters the surface characteristics of ECM scaffolds, including UBM.
- Surface topology and ligand landscape are critical factors influenced by both tissue source and processing.
Conclusions:
- The source tissue significantly determines the inherent surface characteristics of ECM scaffolds.
- Chemical cross-linking is a critical processing step that modifies ECM scaffold surface properties.
- Understanding and controlling these surface characteristics is essential for designing effective tissue engineering scaffolds for clinical applications.
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01:29The Extracellular Matrix
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
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