Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...
NMR Spectroscopy: Spin–Spin Coupling01:08

NMR Spectroscopy: Spin–Spin Coupling

The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved in...
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other axis.
2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The dual-faced pyruvate kinase M2 in tumors: From cytoplasmic metabolic gatekeeper to nuclear transcriptional coactivator.

Biochemical pharmacology·2026
Same author

Age-Specific Contrast Optimization of bSSFP in Fetal Brain.

Magnetic resonance in medicine·2026
Same author

FlexCENT: A frequency-flexible CEST imaging network combining frequency offset encoding and three-dimensional U-Net.

Magnetic resonance letters·2026
Same author

Rapid multi-parametric quantitative MRI via deep learning-based synthetic-to-real reconstruction and 3D SSFP-MOLED imaging.

NeuroImage·2026
Same author

Ultrafast Infant Brain Quantitative MRI Using Overlapping-Echo Acquisition with Volumetric Physical Simulation of Slice-level Non-Idealities.

IEEE transactions on bio-medical engineering·2026
Same author

Source-Free Active Domain Adaptation for Brain Tumor Segmentation via Mamba and Region-Level Uncertainty.

Brain sciences·2026
Same journal

An upconversion FRET biosensor using ATRP-grafted polymer brushes as a signal amplifier for ultrasensitive detection of CYFRA21-1.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same journal

A chemiluminescence sensor for ciprofloxacin detection based on copper ion and aptamer co-modified magnetic microspheres.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same journal

Application of far-infrared spectroscopy for prediction of silicate mineral content in claystones and clay shales.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same journal

A coumarin-based water-soluble fluorescent probe for tandem detection of Cu<sup>2+</sup> and glutathione with application in bioimaging and real sample analysis.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same journal

Chromone-based thiosemicarbazone and semicarbazone as efficient fluorescent sensors for CrO<sub>4</sub><sup>2-</sup>/ Cr<sub>2</sub>O<sub>7</sub><sup>2-</sup> Ion detection.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same journal

Rapid species authentication and protein prediction of porcini mushrooms using FTIR-2DCOS coupled with deep learning.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
See all related articles

Related Experiment Video

Updated: Jun 19, 2026

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

Intermolecular double-quantum coherence NMR spectroscopy in moderate inhomogeneous fields.

Wen Zhang1, Congbo Cai, Shuhui Cai

  • 1Department of Physics, State Key Laboratory of Physical Chemistry of Solid Surfaces, Xiamen University, Xiamen, Fujian 361005, China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|October 16, 2009
PubMed
Summary
This summary is machine-generated.

High-resolution NMR spectra can be achieved in moderate magnetic field inhomogeneities using intermolecular multiple-quantum coherences (iMQCs) with properly set selective pulses. However, spectral quality depends on sample properties and field inhomogeneity levels.

More Related Videos

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)
10:28

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)

Published on: November 2, 2018

Related Experiment Videos

Last Updated: Jun 19, 2026

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)
10:28

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)

Published on: November 2, 2018

Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Physical Chemistry
  • Spectroscopic Techniques

Background:

  • Intermolecular multiple-quantum coherences (iMQCs) enable high-resolution NMR in inhomogeneous magnetic fields.
  • Selective pulses simplify 2D iMQC spectra but are typically limited to small field inhomogeneities.

Purpose of the Study:

  • To investigate the applicability of high-resolution iMQC methods in moderate magnetic field inhomogeneities.
  • To evaluate the impact of selective pulse excitation range on spectral quality in non-ideal fields.

Main Methods:

  • Utilized the IDEAL-II pulse sequence as a model system.
  • Conducted experimental and simulation studies.
  • Analyzed the influence of selective pulse excitation range.

Main Results:

  • High-resolution NMR spectra are achievable in moderate field inhomogeneities when selective pulse excitation is optimized.
  • Undesirable intermolecular cross-peaks emerge at higher field inhomogeneity levels due to distant dipolar fields.
  • Spectral quality is sample-dependent, influenced by chemical shift distributions and J-coupling networks.

Conclusions:

  • High-resolution iMQC methods are viable in moderate inhomogeneous fields with careful parameter selection.
  • The presence of distant dipolar fields limits spectral quality beyond a certain inhomogeneity threshold.
  • Findings are broadly applicable to selective RF pulse-based high-resolution iMQC techniques.