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RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Nonsense-mediated mRNA Decay

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...

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Related Experiment Video

Updated: Jun 19, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Spliceosomes walk the line: splicing errors and their impact on cellular function.

Shu-Ning Hsu¹, Klemens J Hertel

¹Department of Microbiology & Molecular Genetics, University of California, Irvine, CA, USA.

|October 16, 2009

Summary

This summary is machine-generated.

Alternative splicing in humans generates diverse proteins from single genes, but requires high fidelity. This review covers recent advances in understanding alternative splicing extent and accuracy in the human genome.

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Using the E1A Minigene Tool to Study mRNA Splicing Changes

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Last Updated: Jun 19, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Area of Science:

Molecular Biology
Genetics
Genomics

Background:

Nuclear pre-mRNA splicing is crucial for gene expression in most metazoan organisms.
Alternative splicing significantly expands proteomic diversity in higher eukaryotes by producing multiple protein isoforms from a single gene.
Regulation of alternative splicing influences the temporal and spatial production of protein isoforms, impacting cellular activities.

Purpose of the Study:

To review recent progress in understanding the extent of alternative splicing in the human genome.
To emphasize the importance of splicing fidelity in the context of alternative splicing.
To highlight the role of alternative splicing in modulating cellular functions through protein isoform diversity.

Main Methods:

This study is a review of recent scientific literature.
It synthesizes findings on the prevalence and mechanisms of alternative splicing.
Emphasis is placed on studies investigating splicing accuracy and its implications.

Main Results:

A significant portion of human genes undergo alternative splicing, contributing to proteomic complexity.
The regulation of alternative splicing is critical for cellular function and development.
Ensuring high specificity and fidelity in pre-mRNA splicing is essential for producing functional messenger RNAs.

Conclusions:

Alternative splicing is a widespread phenomenon in the human genome, greatly increasing protein diversity.
Splicing fidelity is a critical aspect of gene expression that requires further investigation.
Understanding alternative splicing patterns and their regulation is key to comprehending cellular activities and disease mechanisms.