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Related Concept Videos

RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Histone Modification02:32

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...

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Related Experiment Video

Updated: Jun 19, 2026

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

Methylation, a new epigenetic mark for protein stability.

Xiao-Dong Yang1, Acacia Lamb, Lin-Feng Chen

  • 1Department of Biochemistry, College of Medicine, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Epigenetics
|October 16, 2009
PubMed
Summary
This summary is machine-generated.

Set9, a lysine methyltransferase, modifies non-histone proteins, impacting their stability. This review highlights recent findings on Set9-mediated methylation and its effects on protein stability.

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A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
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Last Updated: Jun 19, 2026

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
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Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
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A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
09:40

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

Published on: April 28, 2022

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Epigenetics

Background:

  • Histone lysine methylation regulates gene expression.
  • Lysine methyltransferases are increasingly found to modify non-histone proteins.
  • Set9 is a well-studied SET domain-containing lysine methyltransferase.

Purpose of the Study:

  • To summarize recent findings on Set9-mediated lysine methylation.
  • To focus on the effects of Set9 on non-histone protein stability.

Main Methods:

  • Literature review of studies on Set9 and non-histone proteins.
  • Analysis of functional studies investigating Set9-mediated methylation.
  • Synthesis of data on protein stability changes.

Main Results:

  • Set9 targets various non-histone proteins, including transcription factors.
  • Set9-mediated methylation of non-histone proteins leads to distinct biological outcomes.
  • A primary consequence of Set9 action on non-histone proteins is altered protein stability.

Conclusions:

  • Set9 plays a significant role in regulating non-histone protein stability through lysine methylation.
  • Understanding Set9's function is crucial for comprehending epigenetic regulation beyond histones.