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09:38
Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry
Published on: June 26, 2019
Direct Repeat 6 from human herpesvirus-6B encodes a nuclear protein that forms a complex with the viral DNA
Mariane H Schleimann1, Janni M L Møller, Emil Kofod-Olsen
1Department of Medical Microbiology and Immunology, Aarhus University, Aarhus, Denmark.
Plos One
|October 16, 2009
Summary
Human herpesvirus 6B (HHV-6B) dr6 gene expression was studied during infection. The DR6 protein interacts with viral factor p41, suggesting a new role in HHV-6B replication.
Area of Science:
- Virology
- Molecular Biology
- Cell Biology
Background:
- Human herpesvirus 6A (HHV-6A) protein DR7 transforms cells and inhibits p53.
- The HHV-6B homolog is dr6, and p53 is deregulated in both HHV-6A and HHV-6B infections.
Purpose of the Study:
- Characterize dr6 mRNA expression and DR6 protein localization during HHV-6B infection.
- Investigate the interaction of DR6 with viral and cellular proteins.
Main Methods:
- Infection of HCT116 cells with HHV-6B.
- Cycloheximide and phosphonoacetic acid treatment to study gene expression.
- Confocal microscopy and co-immunoprecipitation to analyze protein localization and interactions.
Main Results:
- dr6 mRNA expression showed characteristics of herpesvirus early/late genes.
- DR6 protein was detected in the nucleus, accumulating over time and localizing to viral replication compartments.
- A novel interaction between DR6 and the viral DNA processivity factor p41 was observed.
- DR6 and p53 were found in distinct subcellular locations.
Conclusions:
- DR6 exhibits characteristics of a herpesvirus early/late gene.
- DR6 interacts with viral factor p41 within replication compartments.
- DR6 likely plays a novel role in HHV-6B replication, distinct from p53 interaction.
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