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Production of alpha-interferon by human leukocytes
O N Scheglovitova1, A M Kulieva, T G Orlova
1Gamaleya Research Institute of Epidemiology and Microbiology of the AMS U.S.S.R., Moscow.
Abstract:
We examined the capacity of human leukocytes to produce alpha-interferon subtypes following viral induction. It was shown that the beta-propiolactone inactivated viral inductor stimulated the production of a complete set of native alpha-interferon subtypes. Acid treatment of native interferon at pH 2.0 inactivated the acid-labile portion of alpha-interferon. Its exposure to 37 degrees C for 5 days and repeated acid treatment at pH 2.0 resulted in additional inactivation of some interferon pool fractions. The acid-labile subtypes of human alpha-interferon were formed in a nonadherent mononuclear cell fraction.