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Multiphoton laser scanning microscopy of localized scleroderma.

Kecheng Lu¹, Jianxin Chen, Shuangmu Zhuo

¹Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, China.

Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [And] International Society for Digital Imaging of Skin (ISDIS) [And] International Society for Skin Imaging (ISSI)

|October 17, 2009

Summary

Multiphoton laser scanning microscopy (MPLSM) can distinguish localized scleroderma (LS) skin from healthy skin. This non-invasive imaging technique shows promise for in-clinic diagnosis and treatment monitoring of LS.

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Area of Science:

Dermatology
Biomedical Optics
Medical Imaging

Background:

Localized scleroderma (LS) diagnosis and treatment monitoring require effective clinical methods.
Current diagnostic approaches may lack real-time, non-invasive capabilities.

Purpose of the Study:

To evaluate multiphoton laser scanning microscopy (MPLSM) for in vivo diagnosis of LS.
To assess MPLSM's potential for monitoring LS treatment response.

Main Methods:

MPLSM utilizing two-photon excitation fluorescence (TPEF) and second harmonic generation (SHG) was employed.
Human skin specimens (sclerodermatous and normal) were analyzed.
Quantitative parameters including epidermal thickness, collagen bundle orientation, and SHG/TPEF index were developed.

Main Results:

MPLSM clearly visualized morphological differences between normal and sclerodermatous skin.
Developed quantitative parameters showed significant differences between normal and LS skin (P<0.05).

Conclusions:

MPLSM effectively discriminates between sclerodermatous and normal skin.
The clinical portability of MPLSM offers significant potential for in vivo LS diagnosis.
MPLSM can aid in monitoring patient response to LS treatments.