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A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration
Published on: March 17, 2018
Wound healing effect of Flabellaria paniculata leaf extracts
Joseph A O Olugbuyiro1, K A Abo, O O Leigh
1Department of Chemistry, Covenant University, Canaanland, Ota, Ogun, Nigeria. olugbuyiro@yahoo.com
Aim Of The Study:
This study evaluated wound healing activity of the chloroform and aqueous fractions of the methanolic extract of Flabellaria paniculata leaf on infected wounds in rats as a follow up to an initial study of the crude extract.
Materials And Methods:
Wounds were inflicted on Wistar rats using excision model. Local infection was introduced into rat abdominal wounds with Staphylococcus aureus and Pseudomonas aeruginosa using a 10(8)cells/ml inoculum. Rate of wound healing was assessed by contraction and period of epithelization.
Results:
Chloroform fraction exhibited significant wound healing potency (p<0.05) as compared with controls. The test drug achieved 100% wound contraction on day 14 in non-infected group, on day 16 in Staphylococcus aureus infected group and on day 18 in Pseudomonas aeruginosa group.
Conclusions:
Chloroform extract of Flabellaria paniculata proves to be a potential anti-infective and wound healing agent. Its in vitro antibacterial and in vivo wound healing activities are in good agreement with the local medicinal use of the plant for skin diseases and sores.
Insights
The chloroform fraction of Flabellaria paniculata leaf extract significantly promotes wound healing in rats, demonstrating potent anti-infective and tissue regeneration properties for infected wounds.
Area of Science:
- Pharmacology and Ethnobotany
- Natural Product Chemistry
- Wound Healing Research
Background:
- Flabellaria paniculata is traditionally used for treating skin ailments.
- Previous studies indicated wound healing potential of the crude extract.
- This study investigates specific fractions for enhanced efficacy.
Purpose of the Study:
- To evaluate the wound healing activity of chloroform and aqueous fractions of Flabellaria paniculata leaf extract.
- To assess the efficacy on infected wounds in a rat model.
- To validate traditional medicinal use claims.
Main Methods:
- Excision wound model in Wistar rats.
- Infection induced with Staphylococcus aureus and Pseudomonas aeruginosa.
- Wound healing assessed by contraction rate and epithelization period.
Main Results:
- Chloroform fraction showed significant wound healing potency (p<0.05).
- Achieved 100% wound contraction by day 14 (non-infected), day 16 (S. aureus), and day 18 (P. aeruginosa).
- Demonstrated notable efficacy in infected wound models.
Conclusions:
- Chloroform extract of Flabellaria paniculata is a potential anti-infective and wound healing agent.
- In vitro antibacterial and in vivo wound healing activities support its traditional use.
- Further research into its mechanisms is warranted.
