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Updated: Jun 19, 2026

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

Published on: November 7, 2018

Development of an oligonucleotide-based microarray to detect multiple foodborne pathogens.

Biao Suo¹, Yiping He, George Paoli

¹Joint Sino-US Food Safety Research Center & Bor Luh Food Safety Center, School of Agriculture & Biology, Shanghai Jiao Tong University, 800 Dongchuan Rd., Shanghai 200240, China.

Molecular and Cellular Probes

|October 17, 2009

Summary

This summary is machine-generated.

A novel microarray assay effectively detects multiple foodborne pathogens like E. coli O157:H7 and Salmonella in meat. This method offers high sensitivity for improved food safety and pathogen surveillance.

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Published on: November 7, 2018

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Area of Science:

Food Microbiology
Molecular Diagnostics
Genomics

Background:

Escherichia coli O157:H7, Salmonella enterica, Listeria monocytogenes, and Campylobacter jejuni are key foodborne pathogens causing significant human illness.
Existing pathogen detection methods often lack the effectiveness to identify multiple foodborne pathogens simultaneously.

Purpose of the Study:

To develop a cost-effective and sensitive microarray assay for detecting multiple foodborne pathogens.
To validate the assay's performance in identifying specific pathogens and their co-occurrences in food samples.

Main Methods:

Development of a microarray using 70-mer oligonucleotides targeting four major foodborne pathogens.
Utilizing target-specific DNA amplification and 14-plex PCR prior to microarray analysis.
Fabrication of microarray chips with 12 identical arrays for simultaneous screening of multiple samples.

Main Results:

The assay achieved high sensitivity (1 x 10(-4) ng, approx. 20 copies) and specificity, unambiguously distinguishing all four target pathogens.
Analysis of 39 fresh meat samples revealed contamination in 16 samples by one or two pathogens.
Observed co-occurrences of Salmonella with E. coli O157:H7 and L. monocytogenes in the same samples.

Conclusions:

The developed microarray combined with multiplex PCR is an effective tool for screening single or multiple foodborne pathogens.
The method provides valuable genotypic information related to pathogen virulence, enhancing food safety protocols.
This approach offers a sensitive and specific solution for pathogen detection in food matrices.