Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Contribution of endosomal acidification to antigen processing.

K L McCoy1

  • 1Department of Microbiology and Immunology, MCV, Virginia Commonwealth University, Richmond, VA 23298.

Seminars in Immunology
|July 1, 1990
PubMed
Summary

Acidified endosomes are crucial for antigen processing in antigen-presenting cells. Impaired endosomal acidification in mutant cells significantly reduced their ability to process native antigens for CD4+ T cell responses.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Peripheral vascular disease intervention in patients with end-stage renal disease: few complications in those treated with peritoneal dialysis.

Southern medical journal·2001
Same author

Immunosuppression by arsenic: a comparison of cathepsin L inhibition and apoptosis.

International immunopharmacology·2001
Same author

Immunomodulation by cannabinoids is absent in mice deficient for the cannabinoid CB(2) receptor.

European journal of pharmacology·2000
Same author

Delta(9)-tetrahydrocannabinol selectively increases aspartyl cathepsin D proteolytic activity and impairs lysozyme processing by macrophages.

International journal of immunopharmacology·2000
Same author

Cannabinoid inhibition of the processing of intact lysozyme by macrophages: evidence for CB2 receptor participation.

The Journal of pharmacology and experimental therapeutics·1999
Same author

Use of venous duplex scans to evaluate symptoms of deep vein thrombosis: an analysis of ultrasound usage by various medical specialties.

The American surgeon·1999

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Antigen processing is essential for adaptive immunity, involving the breakdown of antigens into peptides for presentation to T cells.
  • Endosomes are key organelles in antigen processing, but their precise role, particularly the impact of acidification, requires further elucidation.

Purpose of the Study:

  • To investigate the role of endosomal acidification in the processing of native antigens for CD4+ T cell responses.
  • To determine if impaired endosomal acidification affects antigen presentation.

Main Methods:

  • Utilized temperature-sensitive mutant cells with defects in endosomal acidification.
  • Assessed antigen processing and presentation capabilities of mutant and wild-type cells at permissive and non-permissive temperatures.
  • Evaluated antigen internalization and degradation.

Related Experiment Videos

Main Results:

  • Mutant cells incubated at the non-permissive temperature showed significantly impaired processing of various native antigens.
  • These cells could still present peptide fragments, indicating the defect was specific to native antigen processing.
  • Antigen internalization and degradation were not affected, nor was antigen processing in wild-type cells.
  • Mutant cells rapidly recovered antigen processing ability upon return to the permissive temperature.

Conclusions:

  • Acidified early endosomes play a critical role in the processing of native antigens.
  • The findings suggest that endosomal acidification is a key step for efficient antigen presentation to CD4+ T cells.
  • Other acidified organelles may also contribute to antigen processing pathways.