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Related Experiment Videos

Polyamines and transglutaminase actions.

J C Romijn1

  • 1Erasmus University, Dept. of Urology, Rotterdam/The Netherlands.

Andrologia
|January 1, 1990
PubMed
Summary
This summary is machine-generated.

Seminal fluid contains polyamines, crucial for protein crosslinking by transglutaminases (TGases). This study reveals TGase activity and polyamine distribution in rat prostates, suggesting a role in seminal clot formation.

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Polyamines and prostatic cancer.

Biochemical Society transactions·2003

Area of Science:

  • Reproductive Biology
  • Biochemistry
  • Enzymology

Background:

  • Polyamines (putrescine, spermidine, spermine) are secreted by the prostate and found in high concentrations in seminal fluid.
  • The precise physiological role of seminal polyamines remains unclear.
  • Polyamines are hypothesized to be substrates for transglutaminases (TGases), enzymes involved in protein crosslinking.

Purpose of the Study:

  • To investigate the distribution of TGase activity and polyamine content across different rat prostate lobes and seminal vesicles.
  • To explore the potential functional relationship between prostatic TGases and polyamines in seminal fluid.

Main Methods:

  • Analysis of transglutaminase (TGase) activities in rat prostate lobes and seminal vesicles.
  • Quantification of polyamine concentrations in these tissues.

Related Experiment Videos

  • Assessment of protein-bound polyamines.
  • Determination of TGase Km-values for polyamines.

    • Main Results:

    • Highest TGase activities were found in the coagulating gland and dorsolateral prostate, with low activity in the ventral prostate.
    • Conversely, polyamine concentrations were highest in the ventral prostate and low in the coagulating glands.
    • Seminal vesicles showed intermediate TGase activity and low polyamine levels.
    • Protein-bound polyamine levels did not correlate with TGase activity, suggesting an extracellular function.
    • TGase Km-values were lower than seminal polyamine concentrations.

    Conclusions:

    • Prostatic transglutaminase activity and polyamine distribution vary significantly across different prostate lobes.
    • The findings support a regulatory role for polyamines in seminal clot formation, likely through extracellular TGase-mediated crosslinking.
    • Further research is warranted to fully elucidate the physiological significance of this interaction.