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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Enlargement of the Plasma Membrane01:22

Enlargement of the Plasma Membrane

Cell division and enlargement are processes that require precise control. The control ensures that cell division cannot proceed unless the cell has grown to a specific size. A spherical, dividing cell requires an approximately 1.6X increase in its surface area to double its volume. The secretory pathway also has a significant role in cell membrane enlargement. Secretory vesicles that bud off from the Golgi apparatus and later fuse with the plasma membrane during exocytosis are a major source of...

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Updated: Jun 19, 2026

Glycopeptide Capture for Cell Surface Proteomics
10:11

Glycopeptide Capture for Cell Surface Proteomics

Published on: May 9, 2014

Technologies for plasma membrane proteomics.

Stuart J Cordwell1, Tine E Thingholm

  • 1School of Molecular and Microbial Biosciences, The University of Sydney, Australia.

Proteomics
|October 17, 2009
PubMed
Summary
This summary is machine-generated.

This review explores plasma membrane (PM) proteomics, focusing on methods to analyze low-abundance signaling proteins. It highlights techniques for comprehensive PM protein analysis crucial for understanding cellular communication.

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Last Updated: Jun 19, 2026

Glycopeptide Capture for Cell Surface Proteomics
10:11

Glycopeptide Capture for Cell Surface Proteomics

Published on: May 9, 2014

Area of Science:

  • Cellular Biology
  • Proteomics
  • Biochemistry

Background:

  • Cellular signaling relies on rapid, transient protein communication for appropriate responses.
  • Proteomics traditionally analyzes abundant effector proteins, under-representing low-abundance signaling proteins.
  • Plasma membrane (PM) proteins, vital for environmental sensing and signal transduction, are often under-represented in proteomics studies.

Purpose of the Study:

  • To review and appraise proteomics approaches for comprehensive plasma membrane (PM) protein analysis.
  • To address the under-representation of PM proteins in current proteomics research.
  • To identify strategies for studying PM proteins as crucial mediators of cellular signaling pathways.

Main Methods:

  • Review of existing proteomics techniques, with a focus on plasma membrane (PM) protein analysis.
  • Discussion of strategies for PM protein enrichment and solubilization.
  • Exploration of cross-linking and glycoproteomics for PM complex purification prior to proteomic analysis.

Main Results:

  • Phosphoproteomics techniques enable temporal profiling of intracellular signaling pathways.
  • Specific methods are needed to overcome challenges in analyzing low-abundance PM proteins.
  • Advanced strategies like cross-linking and glycoproteomics enhance PM protein purification for proteomic analysis.

Conclusions:

  • Comprehensive analysis of PM proteins is essential for understanding cellular signaling.
  • Developing and refining proteomics approaches is crucial for studying these key signaling mediators.
  • Future research should focus on advanced techniques for effective PM protein characterization.