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Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
Published on: March 2, 2017
[Optimization of porcine embryo vitrification]
Defu Zhang1, Jianjun Dai, Caifeng Wu
1Animal Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China. zhangdefu10@yahoo.com.cn
Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|October 20, 2009
Summary
Optimizing porcine embryo cryopreservation is crucial for swine reproduction. Glass micropipette (GMP) vitrification and zona pellucida thinning enhance embryo survival and cell count, with local breeds improving pregnancy rates.
Area of Science:
- Veterinary Science
- Reproductive Biology
- Cryobiology
Context:
- Porcine embryo cryopreservation is vital for genetic resource preservation and efficient swine reproduction.
- Optimizing vitrification protocols is essential to overcome challenges in preserving pig embryos.
Purpose:
- To compare different cryopreservation methods, tools, zona pellucida thinning, and recipient breeds for efficient porcine embryo cryopreservation.
- To evaluate the impact of glass micropipette (GMP) versus open pulled straw (OPS) vitrification on porcine embryo survival and blastocyst cell number.
Summary:
- Glass micropipette (GMP) vitrification showed higher embryo survival rates (83.8%) and blastocyst cell numbers (53.1) compared to the open pulled straw (OPS) method.
- Thinning the zona pellucida (ZP) did not affect survival rates but significantly increased blastocyst cell numbers in surviving embryos (60.1).
- Using local pig breeds, such as Fengjing sows, as recipients improved pregnancy and embryo efficiency for vitrified-warmed blastocysts.
Impact:
- The study provides optimized vitrification techniques for porcine embryos, enhancing cryopreservation efficiency.
- Findings contribute to improved assisted reproductive technologies in swine breeding programs.
- Successful cryopreservation and transfer of porcine embryos can aid in conserving valuable genetic resources.
