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Published on: August 25, 2018
[YPK1 overexpression resulted salt stress hypersensitivity in Saccharomyces cerevisiae is dependent on TOR1]
Hongping Cao1, Yihui Wang, Yan Zhong
1Key Laboratory of Bio-resource and Eco-environment, Ministry of Education, School of Life Science, Sichuan University, Chengdu 610064, China. chp802800@sina.com
Unlabelled:
Serine/threonine protein kinase YPK1, a homologue of mammalian protein kinase serum and glucocorticoid-inducible kinase (SGK), plays important physiological roles in Saccharomyces cerevisiae. It involves in cell wall maintenance, actin cytoskeleton dynamics, endocytosis, translation during nitrogen starvation and nutrient sensing.
Objective:
In order to further explore the cellular function of YPK1 and the its transduction pathway.
Methods:
We overexpressed YPK1 in wild type and tor1 mutant cells and monitored the cell growth responding to salt stress.
Results:
We found that YPK1 overexpression resulted salt stress hypersensitivity in wild type cells and this hypersensitivity was abolished by tor1 mutant.
Conclusion:
Our results indicate that YPK1 may interact with target of rapamycin (TOR1) to regulate the salt stress response.
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