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In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
Published on: May 21, 2015
Subgingival bacterial colonization profiles correlate with gingival tissue gene expression.
Panos N Papapanou1, Jan H Behle, Moritz Kebschull
1Division of Periodontics, Section of Oral and Diagnostic Sciences, College of Dental Medicine, Columbia University, New York, NY, USA. pp192@columbia.edu
BMC Microbiology
|October 20, 2009
Summary
The specific bacteria in periodontal pockets significantly alter gene expression in surrounding gum tissues. Different bacterial species trigger distinct host responses, impacting periodontitis progression.
Area of Science:
- Oral microbiology
- Host-pathogen interactions
- Molecular biology
Background:
- Periodontitis is a chronic inflammatory disease driven by periodontal pocket microbiota.
- Understanding the link between subgingival bacteria and host gene expression is crucial for periodontitis research.
Purpose of the Study:
- To investigate the association between subgingival bacterial profiles and gene expression in gingival tissues of periodontitis patients.
- To identify specific bacterial species that influence host gene expression patterns.
Main Methods:
- Collected subgingival plaque and gingival tissue samples from 120 periodontitis patients.
- Analyzed bacterial species using checkerboard DNA-DNA hybridization.
- Assessed gene expression using whole-genome microarrays and Gene Ontology analysis.
Main Results:
- Significant variations in differentially expressed genes were observed based on bacterial levels.
- Tannerella forsythia and Porphyromonas gingivalis showed the strongest associations with altered gene expression.
- Cluster analysis revealed distinct gene expression patterns regulated by different bacterial species.
Conclusions:
- Subgingival microbial composition directly influences gene expression in gingival tissues.
- Specific periodontal species possess differential abilities to elicit host responses, offering insights into periodontitis pathogenesis.
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