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Updated: Jun 19, 2026
Isolation and Identification of Bacterial Strains from Skin of Terrestrial Amphibians
Published on: June 17, 2025
An immunological method for quantifying antibacterial activity in Salmo salar (Linnaeus, 1758) skin mucus
Edgar Narvaez1, Jorge Berendsen, Fanny Guzmán
1Laboratorio de Genética e Inmunologìa Molecular, Instituto de Biologìa, Facultad de Ciencias, Pontificia Universidad Católica de Valparaìso, Valparaìso, Chile.
Abstract:
Antimicrobial peptides (AMPs) are a pivotal component of innate immunity in lower vertebrates. The aim of this study was to develop an immunological method for quantifying AMPs in Salmo salar skin mucus. A known antimicrobial peptide derived from histone H1 previously purified and described from S. salar skin mucus (SAMP H1) was chemically synthesized and used to obtain antibodies for the quantification of the molecule via ELISA. Using skin mucus samples, a correlation of bacterial growth inhibition versus SAMP H1 concentration (ELISA) was established. The results provide the first evidence for quantifying the presence of active AMPs in the skin mucus of S. salar through the use of an immunological method.
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