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Updated: Jun 19, 2026

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
Published on: March 5, 2019
DNA-dependent protein kinase is a novel interaction partner for Ets-1 isoforms
Souhaila Choul-li1, Hervé Drobecq, Marc Aumercier
1CNRS UMR 8161, Institut de Biologie de Lille, Institut Pasteur de Lille, Univ Lille Nord de France, IFR 142, BP 447, 1 rue du Pr Calmette, 59021 Lille Cedex, France.
Abstract:
The Ets-1 transcription factor plays an important role in various physiological and pathological processes. These diverse roles of Ets-1 are likely to depend on its interaction partner proteins. We used our previously developed, recombinant biotinylated Ets-1 that conserves native Ets-1 properties to identify new interaction partners. Here, based on results from streptavidin pull-down assays, mass spectrometry and co-immunoprecipitation, we report a novel interaction partner for Ets-1 isoforms: a heterotrimeric complex of DNA-dependent protein kinase (DNA-PK), made up of Ku70, Ku86, and DNA-PK catalytic subunit (DNA-PKcs). Kinase assays performed in vitro showed that DNA-PK phosphorylates the Ets-1 protein. Furthermore, we demonstrated that Ku86, but not Ku70 or DNA-PKcs, down-regulated the transcriptional activity of Ets-1 when analysed using a reporter gene assay. These results illustrate how detecting novel molecular interactions may provide new clues for understanding the diverse functions of Ets-1.
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