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Updated: Jun 19, 2026

Gas Chromatography-Mass Spectrometry Paired with Total Vaporization Solid-Phase Microextraction as a Forensic Tool
Published on: May 25, 2021
Optimisation and validation of programmed temperature vaporization (PTV) injection in solvent vent mode for the
José Angel Gómez-Ruiz1, Fernando Cordeiro, Patricia López
1European Commission, Joint Research Centre, Institute for Reference Materials and Measurements (IRMM), Geel, Belgium.
Abstract:
This paper presents the optimisation of a programmed temperature vaporization solvent vent (PTV-SV) injection gas chromatographic mass spectrometric (GC-MS) method for the analysis of the 15+1 EU-priority PAHs in food extracts. Three operation parameters (vent time, vent flow and vent pressure) were optimised by applying a D-optimal experimental design. Among these variables, vent time showed the highest effect on the analytical response (signal intensity) of the target PAHs. The 15+1 EU-priority PAHs were analysed in solvent solutions and in extracts of fortified sausage. In addition, blank lamb meat extracts were prepared and spiked with the target PAHs prior to GC-MS analysis. The performance of the optimised PTV-SV injection GC-MS method was scrutinised for linearity, precision, matrix effects and robustness. All parameters were found satisfactorily. Compared to PTV injection in splitless mode, the PTV-SV injection method provided an enhancement of sensitivity for all target PAHs. Especially significant was the improvement of the S/N ratios of the compounds with the highest molecular mass.
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