FLAG-tag selective covalent protein labeling via a binding-induced acyl-transfer reaction
Hiroshi Nonaka1, Sho-hei Fujishima, Sho-hei Uchinomiya
1Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Kyoto 615-8510, Japan.
Bioorganic & Medicinal Chemistry Letters
|October 20, 2009
Abstract:
A FLAG tag selective protein labeling method is newly developed in this study. Coupling of the selective binding between synthetic Ni-complex probe and FLAG tag with the acyl transfer reaction enables the site-selective covalent modification of FLAG peptide and FLAG-tag fused protein.
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