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Interaction of yeast eIF4G with spliceosome components: implications in pre-mRNA processing events
Panagiota Kafasla1, J David Barrass, Elizabeth Thompson
1Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK. pk303@cam.ac.uk
RNA Biology
|October 20, 2009
Summary
Yeast eIF4G proteins, Tif4631p and Tif4632p, are found in the nucleus and cytoplasm, interacting with splicing machinery components. Deletion of an eIF4G isoform impairs pre-mRNA processing, suggesting a role in nuclear RNA metabolism.
Area of Science:
- Molecular Biology
- Yeast Genetics
- RNA Metabolism
Background:
- Eukaryotic translation initiation factor eIF4G's role in nuclear RNA metabolism is known in mammalian cells.
- The conservation of this function in yeast Saccharomyces cerevisiae remains to be investigated.
Purpose of the Study:
- To investigate the role of yeast eIF4G homologues, Tif4631p and Tif4632p, in nuclear RNA metabolism.
- To determine if the nuclear functions of eIF4G are conserved between mammals and yeast.
Main Methods:
- In vitro and in vivo assays were employed to study yeast eIF4G.
- Protein-RNA and protein-protein interaction studies were conducted.
- Gene deletion and analysis of pre-mRNA processing were performed.
Main Results:
- Yeast eIF4G homologues (Tif4631p, Tif4632p) localize to both nucleus and cytoplasm.
- These proteins interact with U1 snRNA and splicing factors Snu71p and Prp11p.
- Deletion of an eIF4G isoform leads to accumulation of unspliced pre-mRNA.
Conclusions:
- Yeast eIF4G homologues are involved in pre-mRNA processing.
- These findings suggest a conserved role for eIF4G in nuclear RNA metabolism across eukaryotes.
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