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Updated: Jun 19, 2026

Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy
Published on: May 11, 2018
Adaptive harmonic generation microscopy of mammalian embryos
Alexander Jesacher1, Anisha Thayil, Kate Grieve
11Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, UK.
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Adaptive optics is implemented in a harmonic generation microscope using a wavefront sensorless correction scheme. Both the second- and third-harmonic intensity signals are used as the optimization metric. Aberration correction is performed to compensate both system- and specimen-induced aberrations by using an efficient optimization routine based upon Zernike polynomial modes. Images of live mouse embryos show an improved signal level and resolution.

