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Related Experiment Video

Updated: Jun 19, 2026

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

A highly Li(+)-selective glass optode based on fluorescence ratiometry.

Yosuke Ando¹, Yuki Hiruta, Daniel Citterio

¹Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan.

|October 20, 2009

Summary

This study developed a novel optical sensor for lithium ions (Li(+)) using a specialized fluorescent material. This durable and accurate sensor is suitable for medical analysis of lithium levels.

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$Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing$

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

Published on: March 13, 2013

Area of Science:

Analytical Chemistry
Materials Science
Biomedical Engineering

Background:

Accurate monitoring of lithium ion (Li(+)) concentrations is crucial for medical applications, particularly in treating bipolar disorder.
Existing Li(+) sensing methods may suffer from interference, limited sensitivity, or lack of durability.
Development of selective and robust optical sensors is needed for reliable Li(+) detection.

Purpose of the Study:

To prepare and characterize a novel single-excitation, dual-emission ratiometric optical Li(+)-sensing device.
To evaluate the sensor's selectivity, sensitivity, reversibility, and stability for Li(+) detection.
To assess the sensor's performance in real human serum samples for medical analysis.

Main Methods:

Synthesis and design of a highly Li(+)-selective fluoroionophore (KBL-01) incorporating a 14-crown-4 ether binding site and a boron-dipyrromethene fluorophore.
Covalent immobilization of the fluoroionophore onto a porous glass support using a silane-coupling agent to create a Li(+)-selective glass optode.
Characterization of the optode's optical properties, including dual fluorescence emission response, and evaluation of its performance in pseudo-serum and spiked human serum.

Main Results:

The developed Li(+)-selective glass optode exhibits a ratiometric fluorescence response to varying Li(+) concentrations.
The sensor demonstrates high selectivity, with signals independent of interfering cations (Na(+), K(+), Mg(2+), Ca(2+)) and pH.
The sensor shows reversible response, good repeatability, light stability, and accurate performance in spiked real human serum.

Conclusions:

The novel Li(+)-selective glass optode, based on KBL-01 fluoroionophore, is a highly sensitive and accurate device for Li(+) sensing.
The ratiometric detection mechanism ensures signal stability and independence from environmental fluctuations.
This durable optode holds significant potential for reliable medical analyses of lithium levels in biological samples.