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Updated: Jun 19, 2026

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Complete nucleotide sequence of the Toledo isolate of turnip ringspot virus
Sushant Khandekar1, Jie He, Scott Leisner
1Department of Biological Sciences, The University of Toledo, Toledo, OH 43606, USA.
Abstract:
The complete genomic sequence of the Toledo isolate of the comovirus, turnip ringspot virus (TuRSV), was found to consist of 2 polyadenylated RNAs. RNA 1 is 6082 nucleotides long and encodes a single predicted polypeptide of 1860 amino acids. The predicted RNA 1 polyprotein contains the polypeptides for viral replication and proteolytic processing. RNA 2, that is 3985 nucleotides long, codes for a single predicted 1095 amino acid polypeptide containing the movement and coat proteins. Phylogenetic analysis indicates that TuRSV is most closely related to radish mosaic virus, and these crucifer-infecting pathogens form a distinct clade within the comoviruses.
