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Published on: September 9, 2020
Cell-free plasma DNA during Hemodialysis
Sylvie Opatrna1, Jan Wirth, Marie Korabecna
1Department of Medicine I, Charles University, Medical School and Teaching Hospital, Plzen, Czech Republic. opatrna@fnplzen.cz
Cell-free plasma DNA (cfDNA) levels during hemodialysis (HD) are inconsistent after short or long intervals. Changes in cfDNA during HD did not correlate with standard markers but showed a link with diuresis.
Area of Science:
- Nephrology
- Biochemistry
- Molecular Biology
Background:
- Cell-free plasma DNA (cfDNA) is a marker of hemodialysis (HD) membrane biocompatibility.
- cfDNA levels increase during HD, primarily from apoptotic leukocytes.
Purpose of the Study:
- To investigate cfDNA changes during two consecutive high-flux polysulphone HD sessions.
- To compare cfDNA behavior after long (HD-L) versus short (HD-S) interdialytic intervals.
Main Methods:
- 17 HD patients were studied.
- cfDNA and leukocyte counts were measured pre-HD and at 30 and 240 minutes post-HD using real-time PCR.
- Correlations with clinical and biochemical parameters were assessed.
Main Results:
- No significant difference in pre-HD cfDNA between HD-S and HD-L.
- A significant cfDNA increase was observed at 240 min in HD-L, but not HD-S.
- Leukocyte count correlated with pre-HD-S cfDNA; diuresis magnitude associated with cfDNA levels in HD-S.
Conclusions:
- cfDNA behavior during HD is inconsistent after varying interdialytic intervals.
- Laboratory and clinical parameters do not fully explain cfDNA changes during HD.
- The relationship between plasma cfDNA and diuresis warrants further investigation.
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