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Related Experiment Video

Updated: Jun 19, 2026

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers (MADM)
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Quantitative analysis of patch patterns in mosaic tissues with ClonalTools software.

Richard L Mort1

  • 1Division of Reproductive and Developmental Sciences, Genes and Development Group, The University of Edinburgh, Edinburgh, UK. richardm@hgu.mrc.ac.uk

Journal of Anatomy
|October 21, 2009
PubMed
Summary
This summary is machine-generated.

ClonalTools offers a semi-automated method for analyzing mosaic tissues, improving speed and ease for developmental lineage studies. This free ImageJ tool provides quantitative measurements of clonal patterns, overcoming subjective limitations of manual analysis.

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Area of Science:

  • Developmental Biology
  • Image Analysis
  • Quantitative Biology

Background:

  • Quantitative analysis of mosaic tissues is crucial for tracking developmental lineages.
  • Existing analytical techniques are often subjective and time-consuming.
  • There is a need for efficient and objective methods to analyze mosaic patterns.

Purpose of the Study:

  • To introduce ClonalTools, a semi-automated image analysis method for quantifying mosaic patterns.
  • To provide a flexible and customizable toolset for researchers.
  • To improve the ease and speed of analyzing developmental lineages.

Main Methods:

  • ClonalTools is a free, customizable toolset for ImageJ, an open-source image analysis package.
  • It analyzes circular, polygonal, or linear mosaic arrays, measuring positive and negative patch numbers and widths.
  • Results are corrected for random clumping and cell type contribution differences.

Main Results:

  • ClonalTools was successfully applied to mouse corneal epithelium, adrenal cortex, and retinal neurite outgrowth.
  • Quantitative validation using a published corneal epithelium study showed no significant difference from manual analysis.
  • The semi-automated method significantly improves ease and speed of use over previous techniques.

Conclusions:

  • ClonalTools provides a rapid and objective method for quantitative analysis of mosaic tissue patterns.
  • This tool facilitates the study of developmental lineages across various biological systems.
  • It represents a significant advancement in the efficiency of clonal analysis.