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Updated: Jun 19, 2026

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
07:50

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

CHD5 is down-regulated through promoter hypermethylation in gastric cancer

Xian Wang1, Kenneth K K Lau, Leo K Y So

  • 1Department of Biology and Chemistry, City University of Hong Kong, Hong Kong SAR, China. wangx118@yahoo.com

Journal of Biomedical Science
|October 21, 2009
PubMed
Abstract

Insights

Chromodomain helicase DNA binding protein 5 (CHD5) acts as a tumor suppressor gene in gastric cancer. Its expression is epigenetically silenced by promoter methylation, leading to uncontrolled cell growth.

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • Nonhistone chromosomal proteins, including chromodomain helicase DNA binding protein 5 (CHD5), are crucial for DNA replication, repair, and gene expression regulation.
  • Deregulation of these proteins is linked to various diseases, notably cancer.
  • CHD5, a novel tumor suppressor gene (TSG), promotes transcription of p19ink4a and p16arf and is frequently deleted in tumors due to its location at 1p36.

Purpose of the Study:

  • To investigate the role of CHD5 in gastric cancer, the second most prevalent cancer globally.
  • To determine if CHD5 functions as a tumor suppressor in gastric cancer and the mechanisms underlying its potential inactivation.

Main Methods:

  • Quantitative RT-PCR was used to assess CHD5 expression in gastric cancer cell lines.
  • Methylation-specific PCR and bisulfite genome sequencing were employed to evaluate CHD5 promoter methylation.
  • Colony formation assays were conducted to test the impact of CHD5 on gastric cancer cell growth.

Main Results:

  • CHD5 expression was significantly down-regulated in all tested gastric cancer cell lines (100%, 7/7).
  • Promoter methylation of CHD5 was detected in all seven gastric cancer cell lines and in 73% (11/15) of primary gastric carcinoma tissues.
  • Pharmacological demethylation restored CHD5 expression, and ectopic CHD5 expression inhibited gastric cancer cell growth.

Conclusions:

  • CHD5 functions as a tumor suppressor gene in gastric cancer.
  • Epigenetic silencing via promoter methylation is a key mechanism for CHD5 down-regulation in gastric cancer.
  • Restoring CHD5 expression can inhibit gastric cancer cell proliferation.

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