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Sampling nucleotide diversity in cotton.

Allen Van Deynze1, Kevin Stoffel, Mike Lee

  • 1Seed Biotechnology Center, University of California, 1 Shields Ave, Davis, CA, USA. avandeynze@ucdavis.edu

BMC Plant Biology
|October 21, 2009
PubMed
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This study developed a new strategy to efficiently characterize genomic variation, including SNPs and indels, within cotton species. This research provides valuable mapped DNA markers for cotton breeding and genetic improvement.

Area of Science:

  • Plant Genomics
  • Crop Science
  • Molecular Genetics

Background:

  • Cultivated cotton (Gossypium hirsutum and G. barbadense) are key fiber crops.
  • Genomic studies are limited by low variation within species.
  • Novel alleles are being introgressed into G. hirsutum from wild relatives.

Purpose of the Study:

  • To develop an efficient strategy for characterizing genomic variation (SNPs and indels) in cotton.
  • To identify and map polymorphic loci within and among cotton species.
  • To provide mapped DNA markers for cotton breeding and genetic research.

Main Methods:

  • Genotyping of over 1000 SNPs and 279 indels across 270 loci.
  • Analysis of a diverse panel of 24 cotton lines from six species.

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  • Genetic mapping of over 200 loci in a core mapping population (TM-1 x 3-79).
  • Main Results:

    • Characterization of SNP and indel diversity at 270 single-copy polymorphic loci in cotton.
    • Development of a strategy for SNP discovery, including pre-screening for copy number and polymorphism.
    • Confirmation that A and D genomes in diploid and tetraploid cotton remain distinct, allowing paralog differentiation.

    Conclusions:

    • The developed strategy efficiently characterizes genomic variation in cotton.
    • Mapped DNA markers are provided for intra-specific crosses and introgression of exotic germplasm.
    • This research enhances the understanding of cotton genome structure and diversity.