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Ultrasound-Guided Microinjection into the Mouse Forebrain In Utero at E9.5
Published on: November 13, 2010
A microinjection technique for targeting regions of embryonic and neonatal mouse brain in vivo
Steve Davidson1, Hai Truong, Yasushi Nakagawa
1Department of Neuroscience, University of Minnesota, Minneapolis, MN 55455, USA.
Brain Research
|October 21, 2009
Abstract:
A simple pressure injection technique was developed to deliver substances into specific regions of the embryonic and neonatal mouse brain in vivo. The retrograde tracers Fluorogold and cholera toxin B subunit were used to test the validity of the technique. Injected animals survived the duration of transport (24-48 h) and then were sacrificed and perfused with fixative. Small injections (
