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Updated: Jun 19, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
Differential regulation of p53 function by protein kinase C isoforms revealed by a yeast cell system
Isabel Coutinho1, Gil Pereira, Mariana Leão
1Laboratório de Microbiologia, REQUIMTE, Faculdade de Farmácia, Universidade do Porto, 4050-047 Porto, Portugal.
Abstract:
The complexity of the mammalian p53 pathway and protein kinase C (PKC) family has hampered the discrimination of the effect of PKC isoforms on p53 activity. Using yeasts co-expressing the human wild-type p53 and a mammalian PKC-alpha, -delta, -epsilon or -zeta, we showed a differential regulation of p53 activity and phosphorylation state by PKC isoforms. Whereas PKC-alpha reduced the p53-induced yeast growth inhibition and cell cycle arrest, PKC-delta and -epsilon enhanced the p53 activity through p53 phosphorylation, and PKC-zeta had no effect on p53. This work identified positive and negative p53 regulators which represent promising pharmacological targets in anti-cancer therapy.
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