A comparative analysis of parvalbumin expression in pinfish (Lagodon rhomboides) and toadfish (Opsanus sp.)

This study examines the role of a myoplasmic protein, parvalbumin, in enhancing muscle relaxation by fishes. Parvalbumin is thought to bind free Ca(2+) during muscle contraction, thereby reducing intracellular [Ca(2+)] in muscle and speeding muscle relaxation by reducing Ca(2+) availability to the troponin complex. We hypothesized that parvalbumin expression is ubiquitously expressed in fish muscle and that its expression levels and role in muscle relaxation would depend on the activity level and the thermal environment of a given fish species. Muscle contractile properties and patterns of parvalbumin expression were examined in pinfish (Lagodon rhomboides) and two species of toadfish (gulf toadfish, Opsanus beta, and oyster toadfish, Opsanus tau). Unlike another sparid (sheepshead), the active swimming pinfish does not express parvalbumin in its slow-twitch red muscle. However, both sheepshead and pinfish have relatively high levels of parvalbumin in their myotomal white muscle. Gulf toadfish from the Gulf of Mexico expressed higher levels of parvalbumin and had faster muscle relaxation rates than oyster toadfish from more northern latitudes. The faster muscle of gulf toadfish also expressed relatively more of one parvalbumin isoform, suggesting differences in the binding properties of the two isoforms observed in toadfish swimming muscle. Parvalbumin expression and its role in muscle relaxation appear to vary widely in fishes. There are many control points involved in the calcium transient of contracting muscle, leading to a variety of species-specific solutions to the modulation of muscle relaxation.