Related Experiment Video

Updated: Jun 19, 2026

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

IL-27 directly restrains lung tumorigenicity by suppressing cyclooxygenase-2-mediated activities

Ming-Yi Ho1, Shr-Jeng Jim Leu, Guang-Huan Sun

  • 1Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Department of Education and Research, Taipei City Hospital, Taipei, Taiwan, Republic of China.

Journal of Immunology (Baltimore, Md. : 1950)
|October 21, 2009
PubMed

Insights

Interleukin-27 (IL-27) gene transfer inhibits lung cancer growth by reducing tumor cell malignancy and invasion. This non-immune mechanism offers potential for novel lung cancer therapies.

Area of Science:

  • Oncology
  • Immunology
  • Molecular Biology

Background:

  • Interleukin-27 (IL-27) demonstrates anti-tumorigenic properties through antiproliferation, antiangiogenesis, and immune stimulation.
  • The non-immune mechanisms underlying IL-27's suppression of lung cancer growth require further investigation.

Purpose of the Study:

  • To investigate the non-immune mechanisms by which IL-27 suppresses lung cancer growth.
  • To evaluate the therapeutic potential of IL-27 in lung cancer treatment.

Main Methods:

  • Established a single-chain IL-27-transduced murine Lewis lung carcinoma (LLC-1) cell line (LLC-1/scIL-27) for in vivo and in vitro studies.
  • Assessed tumor growth, immune responses (IL-12, IFN-gamma, cytotoxic T cell activity), and molecular markers (COX-2, PGE(2), vimentin) in inoculated mice.
  • Evaluated the effects of recombinant IL-27 (rIL-27) on LLC-1 and human non-small cell lung carcinoma (NSCLC) cell lines, including their migratory and invasive capabilities.

Main Results:

  • LLC-1/scIL-27 inoculation significantly retarded tumor growth in mice.
  • IL-27 suppressed cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE(2)) expression, reduced vimentin levels, and decreased cellular migration and invasion.
  • IL-27 treatment also suppressed PGE(2)-induced vimentin expression and invasiveness in lung cancer cells, with reduced tumor growth observed even in immunodeficient mice.

Conclusions:

  • IL-27 exerts significant anti-tumor effects in lung cancer through non-immune mechanisms, including suppression of COX-2/PGE(2) pathways and reduction of cellular malignancy.
  • These findings highlight IL-27's potential as an effective therapeutic agent for lung cancer.

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Chronic Obstructive Pulmonary Disease-II: Pathophysiology01:20

Chronic Obstructive Pulmonary Disease-II: Pathophysiology

Chronic Obstructive Pulmonary Disease (COPD) pathophysiology is intricate and multifaceted, involving a complex interplay of physiological processes. Understanding these mechanisms is crucial for effectively managing and treating COPD. Here is an in-depth look at the critical elements in the pathophysiology of COPD:
Chronic Inflammation
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...