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Updated: Jun 19, 2026

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens
Published on: February 3, 2020
Antisense oligodeoxynucleotide inhibition as a potent diagnostic tool for gene function in plant biology
Chuanxin Sun1, Haile Ghebramedhin, Anna-Stina Höglund
1Department of Plant Biology & Forest Genetics; Uppsala BioCenter; Swedish University of Agricultural Sciences (SLU); Uppsala, Sweden.
Abstract:
Antisense oligodeoxynucleotide (ODN) inhibition emerges as an effective means for probing gene function in plant cells. Employing this method we have established the importance of the SUSIBA2 transcription factor for regulation of starch synthesis in barley endosperm, and arrived at a model for the role of the SUSIBAs in sugar signaling and source-sink commutation during cereal endosperm development. In this addendum we provide additional data demonstrating the suitability of the antisense ODN technology in studies on starch branching enzyme activities in barley leaves. We also comment on the mechanism for ODN uptake in plant cells.
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