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Related Concept Videos

Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...

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Related Experiment Video

Updated: Jun 19, 2026

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
08:38

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Published on: March 3, 2015

Multicellular development and protein-protein interactions.

Richard B Meagher1, Muthugapatti K Kandasamy, Elizabeth C McKinney

  • 1Department of Genetics; Davison Life Sciences Building; University of Georgia; Athens, Georgia USA.

Plant Signaling & Behavior
|October 21, 2009
PubMed
Summary
This summary is machine-generated.

Gene family expansion drives multicellular development. Coevolution of reproductive actin proteins with specific partners, like profilin and actin depolymerizing factor (ADF/cofilin), is crucial for organ development in plants.

Keywords:
Arabidopsisactin dynamicsactin-binding proteinsactin-depolymerizing factorisovariant specificityprofilinprotein networks

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The MultiBac Protein Complex Production Platform at the EMBL
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Related Experiment Videos

Last Updated: Jun 19, 2026

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
08:38

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Published on: March 3, 2015

The MultiBac Protein Complex Production Platform at the EMBL
13:51

The MultiBac Protein Complex Production Platform at the EMBL

Published on: July 11, 2013

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
07:57

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

Published on: August 21, 2019

Area of Science:

  • Evolutionary developmental biology
  • Molecular and Cellular Biology

Background:

  • Macroevolution of plant and animal organs and tissues may depend on gene family expansion.
  • Actin cytoskeletal proteins and their interacting partners form complex networks crucial for development.

Purpose of the Study:

  • To investigate the coevolution of organ-specific protein-protein interactions.
  • To understand the role of actin isovariants in plant development.

Main Methods:

  • Ectopic expression studies in higher plants.
  • Suppression studies using reproductive and vegetative actin isovariants, profilin, and actin depolymerizing factor (ADF/cofilin).

Main Results:

  • Ectopic expression of reproductive actin in vegetative cells caused aberrant F-actin reorganization and abnormal organ development.
  • Overexpression of vegetative actin had minimal effects.
  • Reproductive profilin or ADF/cofilin suppressed extreme ectopic actin phenotypes, unlike vegetative forms.

Conclusions:

  • Data support the coevolution of organ-specific protein-protein interactions.
  • Understanding the interplay between isovariant networks and organ origination is key to explaining multicellular development.