Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Risk Factors for Sacral Insufficiency Fracture After Pelvic Irradiation for Cervical Cancer.

In vivo (Athens, Greece)·2026
Same author

Inherent tissue homeostasis of the juvenile metaphysis provides a foundation for osteosarcoma development.

Nature communications·2026
Same author

Ultrasound-guided biopsy for thoracic lesions: diagnostic performance, safety, and potential role of contrast-enhanced ultrasound.

Journal of medical ultrasonics (2001)·2026
Same author

Docetaxel-Induced Immune Activation Shows Antitumor Synergy With the Tumor-Targeted CD40 Agonist KK2269.

Cancer science·2026
Same author

NaBr-free TEMPO-catalyzed oxidation of cellulose with sodium dichloroisocyanurate in water at pH 9.

Carbohydrate polymers·2026
Same author

Gradient Optical Response of Au Ellipse Nanopillar Array via Anisotropic Pillar Heights: A Highly Tunable Plasmonic Material toward Biosensing Applications.

ACS nanoscience Au·2026

Related Experiment Video

Updated: Jun 19, 2026

High Throughput Microfluidic Rapid and Low Cost Prototyping Packaging Methods
07:51

High Throughput Microfluidic Rapid and Low Cost Prototyping Packaging Methods

Published on: December 23, 2013

An optimal design method for preventing air bubbles in high-temperature microfluidic devices.

Tsuyoshi Nakayama1, Ha Minh Hiep, Satoshi Furui

  • 1School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa, 923-1292, Japan.

Analytical and Bioanalytical Chemistry
|October 21, 2009
PubMed
Summary

This study introduces a microfluidic polymerase chain reaction (PCR) chip that prevents air bubbles using mineral oil. The optimized chip accurately detects genetically modified maize DNA, showing promise for sensitive diagnostics.

More Related Videos

Development of a Microfluidics-Based Approach for Investigating Microtubule Polymer Mechanics
06:03

Development of a Microfluidics-Based Approach for Investigating Microtubule Polymer Mechanics

Published on: May 30, 2025

A Microfluidic Model of Biomimetically Breathing Pulmonary Acinar Airways
09:39

A Microfluidic Model of Biomimetically Breathing Pulmonary Acinar Airways

Published on: May 9, 2016

Related Experiment Videos

Last Updated: Jun 19, 2026

High Throughput Microfluidic Rapid and Low Cost Prototyping Packaging Methods
07:51

High Throughput Microfluidic Rapid and Low Cost Prototyping Packaging Methods

Published on: December 23, 2013

Development of a Microfluidics-Based Approach for Investigating Microtubule Polymer Mechanics
06:03

Development of a Microfluidics-Based Approach for Investigating Microtubule Polymer Mechanics

Published on: May 30, 2025

A Microfluidic Model of Biomimetically Breathing Pulmonary Acinar Airways
09:39

A Microfluidic Model of Biomimetically Breathing Pulmonary Acinar Airways

Published on: May 9, 2016

Area of Science:

  • Biotechnology
  • Microfluidics
  • Molecular Biology

Background:

  • Polymerase chain reaction (PCR) is vital for diagnostics and research.
  • Microscale PCR chips are key for integrated micro total analysis systems (mu-TAS).
  • Air bubbles in microchannels hinder microfluidic PCR performance.

Purpose of the Study:

  • To develop a microfluidic PCR chip that prevents air bubble formation.
  • To optimize the chip for high-temperature applications and continuous-flow PCR.
  • To quantify genetically modified (GM) maize using the developed chip.

Main Methods:

  • Utilized laminar flow fluid dynamics with mineral oil injection to prevent air bubbles.
  • Optimized microfluidic device parameters including pressure, channel length, and oil volume.
  • Performed quantitative continuous-flow PCR on DNA extracted from GM maize (MON 810) and non-GM maize at varying concentrations.
  • Analyzed DNA amplification signals using a laser-based system.

Main Results:

  • Successfully prevented air bubbles in microchannels using a mineral oil technique.
  • Optimized microfluidic PCR chip demonstrated enhanced performance for high-temperature applications.
  • Quantitative detection of GM maize DNA was achieved with the microfluidic chip.
  • DNA amplification signal intensity correlated directly with the concentration of GM maize DNA.

Conclusions:

  • The developed microfluidic PCR chip effectively prevents air bubbles, enhancing reliability.
  • The optimized chip is suitable for high-temperature PCR and sensitive detection of target DNA.
  • This technology offers a promising platform for quantitative analysis of genetically modified organisms and other diagnostic applications.