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Updated: Jun 19, 2026
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A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors
Published on: September 18, 2013
A developmental study of enteric neuron migration in the grasshopper using immunological probes
1University of Veterinary Medicine Hannover, Division of Cell Biology, Institute of Physiology, Hannover, Germany.
Summary
Nitric oxide (NO) and cyclic guanosine monophosphate (cGMP) signaling are crucial for grasshopper enteric neuron migration. NO-dependent cGMP staining highlights migrating midgut neurons, with distinct patterns in foregut ganglia post-migration.
Area of Science:
- Neuroscience
- Developmental Biology
- Cell Signaling
Background:
- Enteric nervous system (ENS) development involves complex neuronal migration.
- The NO/cGMP signaling pathway is implicated in neuronal function but its role in ENS development is less understood.
Purpose of the Study:
- To investigate the role of NO/cGMP signaling in enteric neuron migration in Locusta migratoria.
- To identify cellular cues involved in enteric neuron guidance during plexus formation.
Main Methods:
- Immunofluorescence staining for cGMP in migrating enteric neurons.
- Time-lapse video microscopy of migrating midgut neurons.
- Analysis of cellular structures and cell adhesion molecules.
Main Results:
- NO-dependent cGMP staining was observed in migrating enteric midgut neurons.
- cGMP immunoreactivity appeared in foregut enteric ganglia after major migratory events.
- Midgut musculature and Fasciclin I were suggested to aid in enteric neuron guidance and fasciculation.
Conclusions:
- NO/cGMP signaling is critical for enteric neuron motility during development.
- Migrating neurons exhibit distinct cGMP expression patterns compared to non-migrating ones.
- The midgut musculature and Fasciclin I play roles in guiding enteric neuron development.
