Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Maintenance of the ES Cell State

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Antiosteoporotic activity of echinacoside in ovariectomized rats.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2013

Same author

Targeted Metabolomics of Serum Acylcarnitines Evaluates Hepatoprotective Effect of Wuzhi Tablet (Schisandra sphenanthera Extract) against Acute Acetaminophen Toxicity.

Evidence-based complementary and alternative medicine : eCAM·2013

Same author

Low molecular weight fucoidan against renal ischemia-reperfusion injury via inhibition of the MAPK signaling pathway.

PloS one·2013

Same author

Promoting the activity of catalysts for the oxidation of water with bridged dinuclear ruthenium complexes.

Angewandte Chemie (International ed. in English)·2013

Same author

Tunable green oxygen barrier through layer-by-layer self-assembly of chitosan and cellulose nanocrystals.

Carbohydrate polymers·2013

Same author

Gastrointestinal effects of resistant starch, soluble maize fibre and pullulan in healthy adults.

The British journal of nutrition·2013

Search research articles

Related Experiment Video

Updated: Jun 19, 2026

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

Published on: June 17, 2016

Random mitotic activities across human embryonic stem cell colonies.

Qiaoling Jin¹, Ryan Duggan, Siva S K Dasa

¹Biosciences Division, Argonne National Laboratory, Lemont, Illinois 60439, USA. qjin@anl.gov

Stem Cells and Development

|October 22, 2009

Summary

Human embryonic stem cell (hES) mitotic activity is not spatially or temporally dependent. Mitotically active cells form random clusters, with overall activity remaining constant around 50% over six days post-passaging.

More Related Videos

Generation of Aggregates of Mouse Embryonic Stem Cells that Show Symmetry Breaking, Polarization and Emergent Collective Behaviour In Vitro

Generation of Aggregates of Mouse Embryonic Stem Cells that Show Symmetry Breaking, Polarization and Emergent Collective Behaviour In Vitro

Published on: November 24, 2015

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Published on: May 29, 2014

Related Experiment Videos

Last Updated: Jun 19, 2026

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

Published on: June 17, 2016

Generation of Aggregates of Mouse Embryonic Stem Cells that Show Symmetry Breaking, Polarization and Emergent Collective Behaviour In Vitro

Generation of Aggregates of Mouse Embryonic Stem Cells that Show Symmetry Breaking, Polarization and Emergent Collective Behaviour In Vitro

Published on: November 24, 2015

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Published on: May 29, 2014

Area of Science:

Stem Cell Biology
Developmental Biology
Cellular Dynamics

Background:

Human embryonic stem cells (hESCs) are crucial for regenerative medicine and developmental studies.
Understanding the spatial and temporal regulation of cell division in hESC colonies is vital for controlling tissue development and differentiation.
Previous studies have not comprehensively assessed the distribution and constancy of mitotic activity within hESC colonies over time.

Purpose of the Study:

To investigate whether distinct physical regions within hESC colonies exhibit varying levels of mitotic activity.
To quantitatively assess mitotic activity at different time points (2, 4, and 6 days) post-cell passaging.
To determine the spatial distribution patterns of actively dividing cells within hESC colonies.

Main Methods:

Systemic and quantitative analysis of hESC colonies.
Assessment of mitotic activity via 5-bromo-2-deoxyuridine (BrdU) incorporation to identify S-phase cells.
Tracking of enhanced green fluorescence protein (EGFP)-expressing cells to observe localized cell proliferation.

Main Results:

Mitotically active cells were observed in randomly distributed clusters across hESC colonies.
Localized proliferation was confirmed by the confined growth patterns of EGFP-expressing cells.
The overall percentage of mitotically active cells remained consistently around 50% throughout the 6-day culture period.

Conclusions:

Mitotic activity in hESC cultures is characterized by random clustering of dividing cells, likely due to local cell deposition.
Mitotic activity levels in hESC colonies are independent of time under the studied culture conditions.
These findings provide insights into the intrinsic proliferative dynamics of hESC colonies.