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Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

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Published on: July 18, 2011

Streptavidin 2D crystal substrates for visualizing biomolecular processes by atomic force microscopy.

Daisuke Yamamoto¹, Naoki Nagura, Saeko Omote

¹Department of Physics, Kanazawa University, Kanazawa, Japan.

Biophysical Journal

|October 22, 2009

Summary

High-speed atomic force microscopy (AFM) requires advanced substrates for dynamic imaging. Streptavidin crystals offer suitable surfaces, enabling observation of real-time molecular processes with AFM.

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Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

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Published on: February 27, 2015

Area of Science:

Biophysics
Materials Science

Background:

Flat substrate surfaces are crucial for atomic force microscopy (AFM) imaging of biological macromolecules.
Advancements in high-speed AFM necessitate improved substrates for dynamic imaging, capable of capturing molecular processes at high temporal resolution (e.g., 30 ms).

Purpose of the Study:

To characterize three types of streptavidin two-dimensional crystals as potential substrates for dynamic AFM imaging.
To evaluate their surface properties, including roughness, uniformity, stability, and resistance to nonspecific protein adsorption.

Main Methods:

Characterization of streptavidin crystal surfaces using AFM.
Assessment of surface roughness, uniformity, stability, and resistance to nonspecific protein adsorption.
Demonstration of substrate utility through dynamic AFM imaging of biological processes.

Main Results:

All three streptavidin crystal types demonstrated resistance to nonspecific protein adsorption.
Differences were observed in surface roughness, uniformity, and stability among the crystal types.
The characterized crystal surfaces proved effective for dynamic AFM imaging.

Conclusions:

Streptavidin two-dimensional crystals are viable substrates for dynamic AFM imaging of biological macromolecules.
While exhibiting some variations, these surfaces facilitate the observation of rapid molecular dynamics, including calcium-induced calmodulin changes, GroES/GroEL interactions, and actin polymerization.