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Development and evaluation of a reverse transcription-loop-mediated isothermal amplification assay for rapid and

A Inomata1, N Kishida, T Momoda

  • 1Department of Environmental Health and Toxicology, Tokyo Metropolitan Institute of Public Health, Shinjuku, Tokyo, Japan. Akiko_Inomata@member.metro.tokyo.jp

Water Science and Technology : a Journal of the International Association on Water Pollution Research
|October 22, 2009
PubMed

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...